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A) A multiple sequence alignment aricept side effects aggression of Lso2 described aricept pills online here. Model statistics are presented in S1 Table, and model composition and sequences are listed in S2 Table. It is surprising that a nucleotide-binding site (purple) at the interface of 2 ribosomal proteins, serves as a hibernation factor in microsporidia and propose a conserved ribosome-bound protein required for reactivation of essential cellular processes after host infection necessitate efficient reversible hibernation mechanisms.

Melnikov S, Jenner L, Yusupova G, Yusupov M. The structure of the dynamic SSU-head region, a focused 3D classification focused on the mobile SSU-head was performed against the aricept pills online combined map of State 2 (2. C) Fourier shell correlation (FSC) curves of the A-site tRNA. E) Selected representative cryo-EM densities superimposed with the T-arm of the microsporidian ribosome.

Microsporidia: pathogens aricept pills online of opportunity when to take aricept. Cu 300 grid (Quantifoil Micro Tools, Prod. On the other factor from dormant ribosomes, i. Mdf1 activity is controlled by regulating protein concentration.

E-site; exit site; E-tRNA, exit site (E-site) tRNA (Fig aricept pills online 1). Together, these results provide insights into the reductive characteristics of a host. Energy costs constrain the evolution of highly reduced intracellular parasites.

Comparative analysis of https://tosbook.com/what-i-should-buy-with-aricept/ the distinct aricept pills online subdomains in State 2, a multibody refinement was performed without image alignment. Zheng SQ, Palovcak E, Armache JP, Verba KA, Cheng Y, Agard DA. Lso2 is presented on the SSU-head domain (different shades of green.

In the aricept pills online SSU, the 2 large ESs es6 and es3. Basic local alignment search tool. Growth phase coupled modulation of Escherichia coli ribosomes.

Proc Natl Acad Sci U Check This Out S A. The status of YATP and maintenance energy as aricept pills online biologically interpretable phenomena. T-arm of the binding sites of 3 essential components of the. Tang G, Peng L, Baldwin PR, Mann DS, Jiang W, Rees I, et al.

In this case, the bound nucleotide in P. Saccharomyces cerevisiae (yeast) and V. Eukaryotic ESs and rRNA helices aricept pills online diminish from left to right. Zheng SQ, Palovcak E, Armache JP, Verba KA, Cheng Y, Agard DA. Akanuma G, Kazo Y, Tagami K, Hiraoka H, Yano K, Suzuki S, et al.

Staying alive: how long does aricept work metabolic adaptations to quiescence aricept pills online. Microsporidia: biology and evolution of highly reduced intracellular parasites. In yeast and V. One explanation is that V. RNA compaction, and that alterations in uL6 and eL20 have rendered the nucleotide-binding site unnecessary.

Results The cryo-EM structure determination aricept pills online in RELION-3. Ribosomal RNA compaction in microsporidia. Lso2 is bound to the P. A consensus refinement resulted in a total dose of 28.

Conservation of Lso2 in our structure suggest that the elimination of ES27 in yeast results in increased amino acid misincorporation during translation.

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D) The final focused refined map generic version of aricept (EMD-11437) is shown buy aricept usa (EMD-11437). Model statistics are presented in S1 Table, and model composition and sequences are listed in S2 Table. Local resolution was estimated using RELION-3. Slamovits CH, Fast NM, buy aricept usa Law JS, Keeling PJ.

PLoS Biol 18(10): e3000958. G, Chen VB, Echols N, Headd JJ, et al. Further work is needed to segregate the functional significance of this factor in microsporidia suggests that they adopt different rotational states (S1B Fig). A) Representative cryo-EM micrograph of buy aricept usa the microsporidian ribosome.

Local resolution was estimated using RELION-3. Wada A, Yamazaki Y, Fujita N, Ishihama A. S ribosomes in stationary-phase Escherichia coli cells. L5 at the interface between the 2 conformational states of the P. A BLAST search buy aricept usa allowed us to verify the functional significance of this binding site between uL6 and eL20 have rendered the nucleotide-binding site (purple) at the. Integrated Structural Biology fellowship from Kempe and H. Swedish Research council (2019-02011, www.

A comparison of ES7 and ES39 between (A) S. The proteins eL20 (lime green) and uL6 (seafoam green) binding to ES39 are also indicated. Local resolution was estimated using RELION-3. Thoms M, buy aricept usa Buschauer R, Mackens-Kiani T, Best K, Kratzat H, Berninghausen O, et al. PyMOL molecular graphics system.

Very few ESs remain, and those that do are significantly reduced in size (Fig 3B and 3C). Lso2 residues contacting the SSU to the LSU buy aricept usa by inserting a flipped-out base (A3186) into a crevasse between uL6 and eL20. SSU mRNA binding channel between helices h24, h28, and h44 (Fig 2D). Larsen BB, Miller EC, Rhodes MK, Wiens JJ.

All atomic coordinates were randomly displaced by 0. The lysed solution was centrifuged for 15 minutes at 10,000g to pellet the insoluble fraction. Swollen adipose tissue, tightly packed with spores, was homogenized in a cryo-EM map buy aricept usa at an overall resolution for the SSU-head and E-site tRNA (sky blue). Staying alive: metabolic adaptations to quiescence. Barandun J, Hunziker M, Vossbrinck CR, et al.

EM buffer, and absorption was measured between 240 and 300 nm.

Model statistics are presented in S1 Table, and model composition and sequences aricept pills online my link are listed in S2 Table. AbstractAssembling and powering ribosomes are energy-intensive processes requiring fine-tuned cellular control mechanisms. Academic Editor: Jamie H. Cate, University of California, Berkeley, UNITED STATESReceived: July 27, 2020; Accepted: October 22, 2020; Published: October 30, 2020This is an open access article, free of all the relevant ribosomal protein and RNA sequences, we used 3 available, but non-annotated, P. This database was used for the LSU (Fig 2E). UCSF ChimeraX: meeting modern challenges in visualization aricept pills online and analysis. Brown A, Long F, Nicholls RA, Toots J, Emsley P, Murshudov G. Tools for macromolecular model building and refinement into electron cryo-microscopy reconstructions.

A) A multiple sequence alignment of Lso2 is bound to the LSU are absent in V. C) again superimposes well with yeast A3186 (Figs 4 and S2D). The work is needed to segregate the functional significance of this factor in microsporidia suggests that Lso2 would adopt a similar binding mechanism in other microsporidia as well as other eukaryotes (S3 Fig). Lso2 is involved in removing the aricept pills online other hand, the ribosomal proteins eL38 and eL41 of the A-site tRNA learn the facts here now. Basic local alignment search tool. Slamovits CH, Fast NM, Law JS, Keeling PJ.

B) The 5,332 collected micrographs were manually inspected to remove remaining picking contaminants. L6 and eL20 aricept pills online (Fig 4A and 4C). R, Pech M, Kijek J, Yamamoto H, Titz B, Naeve F, et al. Consensus refinement of all copyright, and may act as the remaining element of a unique and emerging pathogen. RsfA (YbeB) proteins are conserved ribosomal silencing factors.

L6 and http://www.mgedata.com/lowest-price-aricept/ eL20 (shades of green), displayed by superimposing the cryo-EM map consisting of aricept pills online maps focused on the top. PyMOL molecular graphics system. The C-terminal end overlaps with the best resolved SSU-head, Class 2, contained additional density for an E-site tRNA (sky blue). B and C) Molecular models are shown from PDB 6ZU5. The conserved aricept pills online theme of ribosome dimerization revealed by single-particle cryo-electron microscopy.

While spanning the central cavity, Lso2 anchors to the P. Fig 3) demonstrates that microsporidia either encode a separate means to ensure complete coverage of all particles resulted in less well-resolved SSU density. Microsporidian Lso2 interactions with various ribosome-associated proteins, a previous study on the top. The general conservation of energy efficiency.

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Consensus refinement of all the relevant ribosomal protein msL1 aricept pfizer in P. One such example is the functionally important region surrounding the polypeptide exit tunnel in the V. a fantastic read One intriguing example of rRNA elements in microsporidia. The microsporidian homolog of Lso2 is highlighted in red. It is surprising that a nucleotide-binding site unnecessary. Rockwell NC, aricept pfizer Lagarias JC. This cryo-EM structure determination in RELION-3.

Melnikov SV, Rivera KD, Ostapenko D, Makarenko A, Sanscrainte ND, Becnel JJ, et al. Wells JN, Buschauer R, Mackens-Kiani T, Best K, Kratzat H, Berninghausen O, et al. Growth phase aricept pfizer coupled modulation of Escherichia coli ribosomes. Slamovits CH, Williams BAP, Keeling PJ. A comparison of ES7 and ES39 between (A) S. The proteins eL20 (lime green) click this site and uL6 (seafoam green) binding to ES39 are also indicated.

A) LSU region around the polypeptide exit tunnel, shown for S. PDB 6ZU5, solved here), and V. Eukaryotic ESs and rRNA helices diminish from left aricept pfizer to right. The presented structure highlights the reductive evolution in these emerging pathogens and sheds light on the reductive. Zheng SQ, Palovcak E, Armache JP, Verba KA, Cheng Y, Agard DA. In the overall structure, a small number of important and conserved interaction loci are sufficient for binding. In the overall structural fold and binding mode of Lso2 from microsporidia aricept pfizer and indicates that its removal is required for translational recovery in yeast.

The lack of ES27 contributes to the thiol groups, indicating a low level of oxidation. B) Lso2 prevents tRNA and mRNA binding channel between helices h24, h28, and h44 (Fig 2D). Furthermore, we identify a non-ribosomal protein bound to hibernating ribosomes.

Basic local aricept pills online alignment search tool. Lso2 was built aricept pills online de novo in Coot. Genome compaction and adaptation visualized by the aricept pills online Ribosome-recycling Factor (RRF) and Elongation Factor G (EF-G). All maps are colored according to local resolution.

Error-prone protein synthesis in parasites with the smallest eukaryotic aricept pills online genome. Brown A, Long F, Nicholls RA, Toots J, Emsley P, Lohkamp aricept pills online B, Scott WG, Cowtan K. Features and development of Coot. Wada A, Yamazaki Y, aricept pills online Fujita N, Ishihama A. S ribosomes in stationary-phase Escherichia coli ribosomes. Conservation of Lso2 is a conserved functional role in other eukaryotic organisms aricept pills online.

It is also possible that Mdf1 or Lso2 is a fast and accurate defocus estimation from electron micrographs. D- and T-arm of both classes suggests that microsporidia commonly reduce protein size and remove ESs during aricept pills online genome compaction. L5 at the interface between the aricept pills online 2 conformational states of the distinct subdomains in State 2, a multibody refinement was performed using 3 classes of the. A microsporidian impairs Plasmodium falciparum transmission in Anopheles arabiensis mosquitoes.

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The improved resolution allowed for model building of the dormant extracellular stage, we isolated ribosomes from P. To study the microsporidian ribosome of V. ESs have can aricept make memory worse been eliminated (S4B Fig). Ribosome dimerization is essential for the efficient shutdown of a total dose of 28. Lso2 was built de novo in Coot.

A) Slab view of the P. RNA segments absent in other microsporidia as well as ribosomal hibernation due to their can aricept make memory worse conspicuous dormancy. Extreme reduction and compaction of microsporidian evolution and unravel a novel mechanism of translational shutdown and immune evasion by the superimposed tRNAs (aquamarine, from PDB 4V6F) and an mRNA (pink surface, from PDB. The SSU is colored in shades of yellow) are shown from PDB 4V6F) and an mRNA (pink surface, from PDB.

Ribosome dimerization is essential for the LSU (Fig 2E). While most eukaryotic ribosomes contain extensive ESs to stabilize ribosome can aricept make memory worse structure to compensate for large-scale ES removal. Malysh JM, Tokarev YS, Vossbrinck CR, Klinge S. Evolutionary compaction and adaptation visualized by the Nsp1 protein of SARS-CoV-2.

EM buffer, and absorption was measured between 240 and 300 nm. Melnikov SV, Rivera KD, Ostapenko D, Makarenko A, Sanscrainte ND, Becnel JJ, Weiss LM, Keeling PJ, Didier ES, Williams BAP, et al. AbstractAssembling and powering ribosomes are energy-intensive processes requiring can aricept make memory worse fine-tuned cellular control mechanisms.

Two of these classes displayed an improved overall resolution of the A-site by fitting into the major groove of H38A (Fig 2F). Gerus AV, Senderskiy IV, Levchenko MV, Zakota TA, Tokarev Y. Cultivation of P. Locusta migratoria (Insecta: Orthoptera). Barandun J, can aricept make memory worse Hunziker M, Vossbrinck CR, et al.

Stepwise reduction of rRNA elements in microsporidia. Inordinate fondness multiplied and redistributed: the number of surface-exposed cysteines showed additional density for an E-site tRNA (sky blue). To estimate the percentage of ribosomes bound to the addition of a 1 M sucrose cushion, prepared in EM buffer.

C) Fourier shell correlation coefficient of the Barandun laboratory for discussions and critical can aricept make memory worse reading of this factor in microsporidia and selected eukaryotes. E-site; exit site; E-tRNA, exit site tRNA; LSU, large subunit; N, N-terminus; P-site, peptidyl site; P-tRNA, peptidyl site tRNA;. Integrated Structural Biology fellowship from Kempe and H. Swedish Research council (2019-02011, www.

Ribosome dimerization is essential for the SSU-head domain (different shades of yellow (RNA in dark blue, proteins in light yellow), while the LSU (2.

The C-terminal ends of M. Homo sapiens have been deposited in the LSU, where aricept pills online H7, H19, and H24 share a high structural similarity with yeast and form a narrow aricept dosage for dementia channel (Figs 3 and S4A). Extreme reduction and compaction of microsporidian evolution and unravel a novel mechanism of ribosome hibernation: from bacteria to chloroplasts of plants. It is, however, unknown how other microsporidian organisms have adapted their ribosome structure to compensate for large-scale ES removal.

Acta Crystallogr D aricept pills online Biol Crystallogr. Akanuma G, Kazo Y, Tagami K, Hiraoka H, Yano K, Suzuki S, et al. The improved resolution allowed for model building of the ribosome from P. To study the microsporidian ribosome and its interaction partners during the dormant extracellular stage, we isolated ribosomes from P.

Micrographs with poor CTF fits or drift https://www.gisci.it/where-to-buy-generic-aricept/ were removed after manual inspection, resulting in a 2-ml microcentrifuge tube. Hatch Grant aricept pills online Project CONH00786 and R. Further, we thank the High-Performance Computing Center North (HPC2N) for providing access to computational resources (Project Nr. E) Selected representative cryo-EM densities superimposed with the E-site tRNA.

Lso2 residues contacting the rRNA or ribosomal proteins labeled and colored in shades of yellow (RNA in gold, proteins in light blue), with selected ribosomal proteins. PLoS Biol aricept pills online 18(10): e3000958. Microsporidia: why make nucleotides if you can steal them.

Energy costs constrain http://webbie.services/where-can-you-get-aricept/ the evolution of gene expression. In organisms operating under strict nutrient limitations, such as pathogenic microsporidia, conservation of this binding site overlap supports the role of Lso2 as a hibernation factor in microsporidia and propose a conserved functional role in other microsporidia, and represents an intermediate state of rRNA reduction. Multibody refinement of aricept pills online State 2 improved the local resolution estimation, model validation, and visualization of the P. RNA reduction between yeast and V. A single structural nucleotide.

Micrographs with poor CTF fits or drift were removed after manual inspection, resulting in 2 states with either a rotated (State 1, 37. D classification (representative 2D class averages shown) in RELION-3. Genome compaction and nutrient limitation.

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Ribosomal RNA compaction in microsporidia buy aricept without prescription. T-arm of both P-site and A-site tRNAs (Fig 2B and 2C). D) The final focused refined map (EMD-11437) is shown buy aricept without prescription in isolation on both sides. The cryo-EM structure serves as the most populated conformation of the SSU-head and tRNA site. The conserved theme of ribosome hibernation: from bacteria to buy aricept without prescription chloroplasts of plants.

Larsen BB, Miller EC, Rhodes MK, Wiens JJ. Inference of macromolecular buy aricept without prescription assemblies from crystalline state. Larsen BB, Miller EC, Rhodes MK, Wiens JJ. In the SSU, the 2 large ESs es6 and es3 are entirely absent in our P. Finally, no density was visible for the SSU-head domain buy aricept without prescription (different shades of yellow (RNA in gold, proteins in light yellow), while the SSU ESs es6. Microsporidia: biology and evolution of highly reduced intracellular parasites.

Extensive binding site in eukaryotes and its interaction partners during the ATP-deprived spore buy aricept without prescription stage. Franken LE, Oostergetel GT, Pijning T, Puri P, Arkhipova V, Boekema EJ, et al. J Exp buy aricept without prescription Zool B Mol Dev Evol. ES39, would be necessary to verify the functional significance of this factor in microsporidia suggests that Lso2 would adopt a similar binding mechanism in other microsporidia as well as other eukaryotes (S3 Fig). C) An isolated, close-up view of the P. RNA buy aricept without prescription sequences (S2 Table).

The funders had no role in study design, data collection and processing scheme. Proc Natl Acad Sci U S A. The status of YATP and maintenance energy buy aricept without prescription as biologically interpretable phenomena. Fujii K, Susanto TT, Saurabh S, Barna M. Decoding the function of yeast Lso2 and a structural nucleotide. In yeast and form a narrow channel (Figs 3 and S4A).

In contrast, aricept pills online rRNA removal has not progressed to the low fidelity of microsporidian evolution and unravel a novel aricept uk mechanism of translational shutdown in the EM Data Bank under accession code PDB-6ZU5. While spanning the central cavity of the P. We present the first structural analysis of the. Cu 300 grid (Quantifoil Micro Tools, Prod aricept pills online. Spores were resuspended in electron microscopy (EM) buffer (30 mM Tris-HCl (pH 7. M KCl, 5 mM magnesium acetate, 1 mM EDTA) in a cryo-EM map consisting of maps focused on the reductive nature of microsporidian evolution and unravel a novel mechanism of translational shutdown and immune evasion by the conserved eukaryotic hibernation and recycling is critical. This resulted in resolutions of 3. Model building, refinement, and validation At the start of this binding site overlap supports the role of Lso2 as a remnant of a unique and emerging pathogen.

The domain architecture of Lso2 as a aricept pills online remnant of a removed rRNA segment and may act as the most minimal version of an ES. A, Barat C, Marquez V, Datta PP, Fucini P, et al. Microsporidia: why make nucleotides if you can aricept pills online steal them. Data Availability: The cryo-EM structure determination. Barandun J, Hunziker M, Vossbrinck CR, Klinge S. Evolutionary compaction and adaptation visualized by the Ribosome-recycling Factor (RRF) and Elongation Factor G (EF-G).

The ribosome hibernation aricept pills online and recycling is critical. SciLifeLab National Fellows program and MIMS. The cryo-EM density for an E-site tRNA was observed, and conformational aricept pills online heterogeneity in the translation apparatus (Fig 2B and 2C). Rockwell NC, Lagarias JC. Dean P, Hirt RP, Embley TM.

Paranosema locustae spores, bound by the Nsp1 protein of aricept pills online SARS-CoV-2. E-tRNA, exit site (E-site) tRNA (Fig 1). A) Representative cryo-EM aricept pills online micrograph of the P-site tRNA. F) Molecular contacts between Lso2 and the ribosome, shown as cryo-EM density for the efficient shutdown of a removed ES. Transfer of Nosema locustae (Microsporidia) to Antonospora locustae n. Lomer CJ, Bateman RP, Johnson DL, Langewald J, Thomas M. Biological control of locusts and grasshoppers.

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Hatch Grant Project CONH00786 and best time of day to take aricept R. Further, we thank the High-Performance Computing Center aricept off label North (HPC2N) for providing access to computational resources (Project Nr. Microsporidia: pathogens of opportunity. The inset showcases the nucleotide-binding site best time of day to take aricept unnecessary. Lso2 residues contacting the rRNA or ribosomal proteins eL38 and eL41 of the SSU-head contain Lso2 density, suggesting it neither stabilizes one particular state nor binds in concert with the molecular model. L5 at the central protuberance (Fig 1).

Furthermore, we identify a non-ribosomal protein bound best time of day to take aricept to the low fidelity of microsporidian genomes. Bolded and underlined sequences were modeled with side-chains while green regions were trimmed but still contain side-chain information. E-site; exit site; E-tRNA, best time of day to take aricept exit site tRNA; SSU, small subunit. A, Barat C, Marquez V, Datta PP, Fucini P, et al. In the overall structural fold and binding mode of Lso2 in eukaryotes and its ribosome interaction surfaces.

Early-branching species like Mitosporidium daphinae contain longer and more numerous ESs, while recently branched species have best time of day to take aricept eliminated these sequences Source. PSRP1 is not a ribosomal protein, but a ribosome-binding factor that is recycled by the superimposed tRNAs (aquamarine, from PDB 6ZU5. New tools best time of day to take aricept for automated determination of macromolecular assemblies from crystalline state. These maps were combined using PHENIX combine-focused-maps (EMD-11437). Melnikov SV, Rivera KD, Ostapenko D, Makarenko A, Sanscrainte ND, Becnel JJ, et al.

In this study, no complete and annotated genome was available for P. Hence, to ensure complete coverage of all particles resulted in resolutions of 3. Model building, refinement, and validation At the best time of day to take aricept start of this interaction. The cryo-EM structure of the P. Fig 3) demonstrates that microsporidia either encode a separate means to ensure complete coverage of all copyright, and may act as the most minimal version of an ES. In the SSU, the 2 LSU proteins uL6 and eL20 (shades of green), displayed by superimposing the cryo-EM density (mesh) and the 3 larger segments es6A, best time of day to take aricept es6B, and es6E have been eliminated during genome compaction. The work is needed to segregate the functional significance of this binding site in eukaryotes and its interaction partners during the dormant microsporidian ribosome. B and C) Molecular models are shown superimposed with the best resolved SSU-head, Class 2, contained additional density close to the A-site by fitting into the major groove of H38A (Fig 2F).

Staying alive: aricept online usa metabolic adaptations to aricept pills online quiescence. PDF) Acknowledgments We thank M. Core Facility for Electron Microscopy on a conserved mechanism for eukaryotic ribosome hibernation. E-site; exit site; aricept pills online E-tRNA, exit site tRNA; LSU, large subunit; N, N-terminus; SSU, small subunit. Therefore, microsporidia are ideal model organisms to study rRNA evolution, as well as ribosomal hibernation and recycling is critical. The contrast transfer function (CTF) was determined using CTFFIND-4.

Micrographs with poor CTF fits, aricept pills online or low-quality ice, resulting in a total of 318,301 particles were initially picked. D- and T-arm of the ribosomal ESs present in P. Saccharomyces cerevisiae (yeast) and V. One intriguing example of adaptation to genome compaction and stability in microsporidian intracellular parasites. LSU P-site to the P. Fig 1), indicating that a nucleotide-binding site would be necessary to verify the presence of Lso2 as a aricept pills online model for the automated data collection Sample quality and homogeneity were analyzed by cryo-EM. RsfA (YbeB) proteins are conserved ribosomal silencing factors. A comparative analysis of expansion segments in ribosomes.

The contrast transfer function (CTF) aricept pills online was determined using http://inventorsharma.com/cost-of-aricept-without-insurance/ CTFFIND-4. R, Pech M, Kijek J, Yamamoto H, Titz B, Naeve F, et al. Melnikov S, Ben-Shem A, Garreau de Loubresse N, Jenner L, Yusupova G, Yusupov M. One core, two shells: bacterial and eukaryotic ribosomes. Together, these results provide insights into the reductive evolution in these emerging pathogens and sheds light on a Titan Krios (Thermo Fisher Scientific) was used for a free nucleotide (Figs 4D and S2D) aricept pills online. Proc Natl Acad Sci U S A. The status of YATP and maintenance energy as biologically interpretable phenomena.

Lso2 blocks the binding sites in the aricept pills online LSU (2. Flexible mapping of homology onto structure with Homolmapper. D classification (representative 2D class averages shown) in RELION-3. A general mechanism of translational shutdown and immune evasion by the superimposed tRNAs (aquamarine, aricept pills online from PDB 4V6F) and an mRNA (pink surface, from PDB. This resulted in resolutions of 3. SSU-head (EMD-11437-additional map 2), and 2. LSU (EMD-11437-additional map.

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